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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Fig. 2

Effects of pyruvate stress and CeGNA1 mutation on GlcNAc fermentation. Comparison of extracellular pH (pHex) (a) and intracellular pH (pHin) (b) during fermentation of the control strain BSGN5 and the engineered BSGN12 transformed with the plasmid pP43-cMyc (M-Rm)-CeGNA1 or pP43-cMyc (M-Rm)-CeGNA1-Q155V/C158G, respectively. c Effects of CeGNA1 mutation on cell growth (dry cell weight, DCW), GlcNAc production, and pyruvate accumulation. d SDS-PAGE analysis of the purified wild type (1, CeGNA1) and mutant CeGNA1 (2, CeGNA1-Q155V/C158G). Effects of CeGNA1 mutation on the activity (e) and pH stability (f) of CeGNA1

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