TableĀ 2 scFvĪ² expression and periplasmic secretion titres with DsbA E1 and Piii E5 signal peptides in the BL21(LV2)-pDEST and BL21(DE3)-pET28 strains
From: Tuning recombinant protein expression to match secretion capacity
| Ā | scFvĪ² | |||
|---|---|---|---|---|
Signal peptide | DsbA-E1 | Piii-E5 | ||
BL21 strain | (LV2) | (DE3) | (LV2) | DE3 |
Expression titre mg/L | 36.7 Ā± 10.4 | 25.3 Ā± 3.6 | 101.7 Ā± 31.1 | 6.2 Ā± 0.5 |
Induction control (max/basal) | ā„ā3666 | 17 | ā„ā10,165 | 3 |
Secretion titre mg/L | 26.2 Ā± 7.3 | 4.5 Ā± 0.7 | 17.0 Ā± 0.2 | 1.9 Ā± 0.3 |
OD600 at max | 11.0 Ā± 0.3 | 3.7 Ā± 0.3 | 10.0 Ā± 0.5 | 1.5 Ā± 0.0 |