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Table 2 scFvβ expression and periplasmic secretion titres with DsbA E1 and Piii E5 signal peptides in the BL21(LV2)-pDEST and BL21(DE3)-pET28 strains

From: Tuning recombinant protein expression to match secretion capacity

  scFvβ
Signal peptide DsbA-E1 Piii-E5
BL21 strain (LV2) (DE3) (LV2) DE3
Expression titre
mg/L
36.7 ± 10.4 25.3 ± 3.6 101.7 ± 31.1 6.2 ± 0.5
Induction control
(max/basal)
≥ 3666 17 ≥ 10,165 3
Secretion titre
mg/L
26.2 ± 7.3 4.5 ± 0.7 17.0 ± 0.2 1.9 ± 0.3
OD600 at max 11.0 ± 0.3 3.7 ± 0.3 10.0 ± 0.5 1.5 ± 0.0
  1. Performed in shaker flask induced for 14 h at 30 °C. No basal expression was observed in the BL21(LV2) strains, within the western blot detection limit. Induction control (max/basal) was calculated relative to the lower detection limit