Fig. 6

SDS-PAGE and western blot analysis of Trial 2 fermentation samples. Coomassie stained SDS PAGE (upper pannel) and western blot analysis were performed on both spheroplast (SP) and periplasm (PP) samples of the pDEST-DsbA E1-scFvβ from 0 h and 4 h post-induction. The induction conditions were IPTG (100 µM) and increasing concentrations of PPDA (4, 40, 200 and 400 µM). The scFvβ protein was detected using the Anti-α His antibody. The E. coli anti-RNAP σ70 was used as a control and the signal was only detected in the SP cellular fractions demonstrating the correct cell fractionation procedure. Lysozyme (labelled) was used for the fractionation procedure