Fig. 5

Fed-batch fermentation cell growth, scFv protein yield and location. a, b Trial 1—scFvβ expressed with DsbA-E1 or Piii-E5 signal peptides samples collected at 4 h post-induction. c, d Trial 2—scFvβ or scFvT expressed with DsbA-E1 signal peptide samples collected at 4 h post-induction. The scFv yields plotted against inducer concretion (IPTG/PPDA) fixed IPTG (100 µM) and increasing PPDA concentrations (0, 4, 20, 40, 200, 400 µM) with scFv quantified from western blot analysis in the media (M), periplasm (PP) and spheroplast (SP) fractions. Protein production quantification was performed in technical triplicates