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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Tuning recombinant protein expression to match secretion capacity

Fig. 2

scFvβ expresion control and correlation analysis for codon-optimised signal peptide sequences constructs. a Dose response curves for pENTRY constructs, relative fluorescent units normalized to cell density (RFU/OD600) against inducer concentration for the WT and codon optimised DsbA and Piii signal peptide sequences. b Dose response curves for pDEST constructs, expression yield per cell reported as mg per g dry cell weight. c Linear regression of the pENTRY expression vs. pDEST expression for DsbA-E1 and Piii-E5. d Linear regression of the pENTRY expression vs pDEST secretion for the DsbA E1 and Piii E5. All data was taken from shaker flask expression under different inducer concentrations at 30 °C, 14 h post induction performed in biological triplicates

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