Table 2 Features of Gram-negative bacterial secretion systems to consider when selecting a production platform
From: Developing Gram-negative bacteria for the secretion of heterologous proteins
Secretion system | Number of steps | Secretion tag cleavage | Pathogenic origin | Access to folding chaperones | Secretion system induction scheme |
|---|---|---|---|---|---|
Type I | One | No | Yes | No | Synthetic |
Type II | Two | Unknown | Yes | Yes | Constitutive |
Leaky membrane | Two | No tag needed | No | Yes | Constitutive |
Unknown mechanism | Two | No | No | Yes | Constitutive |
Type III | One | No | Yes | No | Synthetic |
Type V | Two | Partial | Yes | Yes, but unfolded for transport across OM | Synthetic |
Type VIII | Two | No | Yes | Yes | Synthetic |