Table 1 Summary of Gram-negative bacterial secretion systems for heterologous protein production
From: Developing Gram-negative bacteria for the secretion of heterologous proteins
Secretion system | Model organisms | Genetic locus | Model secretion tag | Master regulator |
|---|---|---|---|---|
Type I | Pseudomonas fluorescens | tliDEF | TliA | |
Type I | Escherichia coli | hlyA, hlyB, hlyD | HlyA | |
Type II | E. coli | gsp | Unknown | |
Generalized two-step | E. coli | N/A | YebF | |
Type III | Salmonella enterica | SPI-1 | SptP | HilA |
Type III | S. enterica | fli, flhDC | FlgM (flagellar) | FlhD4C2 (flagellar) |
Type III | Enteropathogenic E. coli (EPEC) | Locus of enterocyte effacement (LEE) | EspA | Ler |
Type III | Shigella flexneri | Shigella virulence plasmid | OspB | VirB, VirF |
Type III | Yersinia enterocolitica | pYV virulence plasmid | YopE | VirF |
Type V | E. coli | tps | Pet | |
Type VIII | E. coli | csg | CsgA | CsgD (not necessary for synthetic induction) |