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Table 6 Calculated NADPH, NADH and ATP production and consumption (mmol/g biomass) based on \(^{13}\hbox {C}\)-MFA fluxes and biomass and protein synthesis requirements

From: Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein

 

S. lividans with pIJ486

S. lividans with pIJ486-vsi-celA

Production

Consumption

\({{\Delta }}\)

Production

Consumption

\({{\Delta }}\)

NADPH

11.6

\(-\) 13.3

\(-\)1.7

25.2

\(-\) 13.6

11.7

NADH

43.1

0.0

43.1

77.3

0.0

77.3

ATP

33.9

\(-\) 52.3

\(-\) 18.3

58.9

\(-\) 61.7

\(-\)2.9

mATP

43.8

  

130.6

  

NGAM

8.4

  

15.0

  
  1. A balance is obtained by diverting differences (\(\Delta \)) to maintenance ATP (mATP), returning the non-growth associated ATP maintenance (NGAM) (mmol gDW−1 h−1)