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Table 6 Calculated NADPH, NADH and ATP production and consumption (mmol/g biomass) based on \(^{13}\hbox {C}\)-MFA fluxes and biomass and protein synthesis requirements

From: Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein

  S. lividans with pIJ486 S. lividans with pIJ486-vsi-celA
Production Consumption \({{\Delta }}\) Production Consumption \({{\Delta }}\)
NADPH 11.6 \(-\) 13.3 \(-\)1.7 25.2 \(-\) 13.6 11.7
NADH 43.1 0.0 43.1 77.3 0.0 77.3
ATP 33.9 \(-\) 52.3 \(-\) 18.3 58.9 \(-\) 61.7 \(-\)2.9
mATP 43.8    130.6   
NGAM 8.4    15.0   
  1. A balance is obtained by diverting differences (\(\Delta \)) to maintenance ATP (mATP), returning the non-growth associated ATP maintenance (NGAM) (mmol gDW−1 h−1)