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Table 5 Estimated yields per 100 mmol glucose for the CelA-producing and the empty-plasmid S. lividans based on concentration measurements from repeated experiments in minimal medium with glucose (MMGLC)

From: Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein

Yield (per 100 mmol glucose)* S. lividans with pIJ486 S. lividans with pIJ486-vsi-celA
Experiment 1 Experiment 2 Experiment 1 Experiment 2
DW (g)* 7.67 (\(\pm \,0.26\)) 9.31 (\(\pm \,0.19\)) 4.96 (\(\pm \,0.17\)) 6.99 (\(\pm \,0.21\))
\(\hbox {CO}_2\) (mmol)* 213.38 (\(\pm \,7.47\)) 213.55 (\(\pm \,88.31\)) 246.07 (\(\pm \,13.14\)) 182.61 (\(\pm \,9.67\))
ACE (mmol)* 8.71 (\(\pm \,3.33\)) 5.97 (\(\pm \,4.07\)) 22.64 (\(\pm \,0.60\)) 28.48 (\(\pm \,3.73\))
AKG (mmol)* 0.11 (\(\pm \,0.01\)) 0.44 (\(\pm \,0.27\)) 0.24 (\(\pm \,0.04\)) 0.47 (\(\pm \,0.04\))
LAC (mmol)* 1.36 (\(\pm \,0.24\)) 1.25 (\(\pm \,0.27\)) 1.37 (\(\pm \,0.12\)) 1.62 (\(\pm \,0.09\))
PYR (mmol)* 0.11 (\(\pm \,0.03\)) 0.86 (\(\pm \,0.57\)) 8.56 (\(\pm \,0.28\)) 16.96 (\(\pm \,2.24\))
SUCC (mmol)* 0.10 (\(\pm \,0.01\)) 0.00 (\(\pm \,0.00\)) 0.18 (\(\pm \,0.03\)) 0.15 (\(\pm \,0.01\))
Protein (mg)* 59.59 (\(\pm \,8.16\)) 94.30 (\(\pm \,4.34\)) 118.43 (\(\pm \,12.56\)) 119.89 (\(\pm \,6.38\))
CelA (mg)* nd nd 20.47 (\(\pm \,5.66\)) 16.96 (\(\pm \,2.25\))
C recovery 0.89 (\(\pm \,0.02\)) 0.99 (\(\pm \,0.15\)) 0.86 (\(\pm \,0.02\)) 0.95 (\(\pm \,0.03\))
  1. Standard deviations are given between brackets and the carbon recovery per experiment is calculated. The carbon content of the biomass was determined through elemental analysis, and found to be 35.17 (\(\pm 0.45\)) mmol/gDW
  2. Dry weight (DW), carbon dioxide (\(\hbox {CO}_2\)), acetic acid (ACE), \(\alpha \)-ketoglutaric acid (AKG), lactic acid (LAC), pyruvic acid (PYR), succinic acid (SUCC), total secreted proteins (Protein), and secreted CelA, nd not determined