Table 5 Estimated yields per 100 mmol glucose for the CelA-producing and the empty-plasmid S. lividans based on concentration measurements from repeated experiments in minimal medium with glucose (MMGLC)
From: Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
Yield (per 100 mmol glucose)* | S. lividans with pIJ486 | S. lividans with pIJ486-vsi-celA | ||
|---|---|---|---|---|
Experiment 1 | Experiment 2 | Experiment 1 | Experiment 2 | |
DW (g)* | 7.67 (\(\pm \,0.26\)) | 9.31 (\(\pm \,0.19\)) | 4.96 (\(\pm \,0.17\)) | 6.99 (\(\pm \,0.21\)) |
\(\hbox {CO}_2\) (mmol)* | 213.38 (\(\pm \,7.47\)) | 213.55 (\(\pm \,88.31\)) | 246.07 (\(\pm \,13.14\)) | 182.61 (\(\pm \,9.67\)) |
ACE (mmol)* | 8.71 (\(\pm \,3.33\)) | 5.97 (\(\pm \,4.07\)) | 22.64 (\(\pm \,0.60\)) | 28.48 (\(\pm \,3.73\)) |
AKG (mmol)* | 0.11 (\(\pm \,0.01\)) | 0.44 (\(\pm \,0.27\)) | 0.24 (\(\pm \,0.04\)) | 0.47 (\(\pm \,0.04\)) |
LAC (mmol)* | 1.36 (\(\pm \,0.24\)) | 1.25 (\(\pm \,0.27\)) | 1.37 (\(\pm \,0.12\)) | 1.62 (\(\pm \,0.09\)) |
PYR (mmol)* | 0.11 (\(\pm \,0.03\)) | 0.86 (\(\pm \,0.57\)) | 8.56 (\(\pm \,0.28\)) | 16.96 (\(\pm \,2.24\)) |
SUCC (mmol)* | 0.10 (\(\pm \,0.01\)) | 0.00 (\(\pm \,0.00\)) | 0.18 (\(\pm \,0.03\)) | 0.15 (\(\pm \,0.01\)) |
Protein (mg)* | 59.59 (\(\pm \,8.16\)) | 94.30 (\(\pm \,4.34\)) | 118.43 (\(\pm \,12.56\)) | 119.89 (\(\pm \,6.38\)) |
CelA (mg)* | nd | nd | 20.47 (\(\pm \,5.66\)) | 16.96 (\(\pm \,2.25\)) |
C recovery | 0.89 (\(\pm \,0.02\)) | 0.99 (\(\pm \,0.15\)) | 0.86 (\(\pm \,0.02\)) | 0.95 (\(\pm \,0.03\)) |