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Table 6 Results from small-scale pulsed fed-batch cultivations of E. coli W/445_Ediss using molasses minimal medium

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Pulse rS [g l−1 h−1] qS [g g−1 h−1] rdiol [g l−1 h−1] qdiol [g g−1 h−1] Ydiol/S [g g−1] Biomass [g l−1]
1st 3.90 ± 0.03 0.57 ± 0.01 1.54 ± 0.27 0.23 ± 0.04 0.39 ± 0.07 6.82 ± 0.08
2nd 4.18 ± 0.56 0.49 ± 0.05 1.80 ± 0.07 0.21 ± 0.02 0.44 ± 0.08 8.44 ± 0.32
3rd 1.77 ± 0.30 0.20 ± 0.03 0.88 ± 0.07 0.10 ± 0.01 0.50 ± 0.04 8.73 ± 0.32
Total 3.01 ± 0.22 0.38 ± 0.02 1.31 ± 0.06 0.16 ± 0.01 0.44 ± 0.03 7.99 ± 0.20
  1. Volumetric and specific substrate uptake rates, rS and qS, volumetric and specific diol (2,3-butanediol + acetoin) formation rates, rdiol and qdiol, diol (2,3-butanediol + acetoin) yield and average biomass concentration are given individually for each pulse and for the total production phase
  2. Results are given as mean value of three replicates ± standard deviation