Skip to main content

Table 6 Results from small-scale pulsed fed-batch cultivations of E. coli W/445_Ediss using molasses minimal medium

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Pulse

rS [g l−1 h−1]

qS [g g−1 h−1]

rdiol [g l−1 h−1]

qdiol [g g−1 h−1]

Ydiol/S [g g−1]

Biomass [g l−1]

1st

3.90 ± 0.03

0.57 ± 0.01

1.54 ± 0.27

0.23 ± 0.04

0.39 ± 0.07

6.82 ± 0.08

2nd

4.18 ± 0.56

0.49 ± 0.05

1.80 ± 0.07

0.21 ± 0.02

0.44 ± 0.08

8.44 ± 0.32

3rd

1.77 ± 0.30

0.20 ± 0.03

0.88 ± 0.07

0.10 ± 0.01

0.50 ± 0.04

8.73 ± 0.32

Total

3.01 ± 0.22

0.38 ± 0.02

1.31 ± 0.06

0.16 ± 0.01

0.44 ± 0.03

7.99 ± 0.20

  1. Volumetric and specific substrate uptake rates, rS and qS, volumetric and specific diol (2,3-butanediol + acetoin) formation rates, rdiol and qdiol, diol (2,3-butanediol + acetoin) yield and average biomass concentration are given individually for each pulse and for the total production phase
  2. Results are given as mean value of three replicates ± standard deviation