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Table 5 Screening results for shake flask cultivations of E. coli W/445_Ediss after 48 h using 5% (w/v) carbon source in minimal medium

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Substrate Substrate [g l−1] Biomass [g l−1] 2,3-Butanediol [g l−1] Acetoin [g l−1] Ydiol/S [g g−1] YX/S [g g−1]
Glucose 50.09 ± 0.01 4.29 ± 0.16 3.44 ± 0.10 16.78 ± 0.24 0.40 ± 0.01 0.09 ± 0.01
Glucose + fructose 51.90 ± 0.08 5.56 ± 0.33 9.41 ± 1.76 10.92 ± 1.92 0.39 ± 0.01 0.11 ± 0.01
Fructose 51.30 ± 0.28 6.98 ± 0.50 4.57 ± 0.61 14.03 ± 0.70 0.36 ± 0.04 0.14 ± 0.01
Xylose 23.24 ± 2.00 3.01 ± 0.10 5.17 ± 0.87 1.11 ± 0.09 0.27 ± 0.02 0.06 ± 0.01
Arabinose 49.24 ± 0.28 5.04 ± 0.05 12.92 ± 0.02 5.65 ± 0.08 0.38 ± 0.01 0.10 ± 0.01
Mannose 46.25 ± 1.32 5.82 ± 0.02 2.56 ± 0.26 14.74 ± 0.29 0.37 ± 0.01 0.13 ± 0.01
Sucrose 38.23 ± 0.72 7.25 ± 0.16 4.51 ± 1.12 5.45 ± 1.55 0.26 ± 0.01 0.19 ± 0.01
Molasses 52.97 ± 0.24 10.89 ± 0.14 3.04 ± 0.94 15.31 ± 1.07 0.35 ± 0.01 0.21 ± 0.01
  1. Consumed substrate, formed biomass and products as well as the diol yield (Ydiol/S) and biomass yield (YX/S) are shown
  2. Results are shown as the mean value of three replicates ± standard deviation