Skip to main content

Table 5 Screening results for shake flask cultivations of E. coli W/445_Ediss after 48 h using 5% (w/v) carbon source in minimal medium

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Substrate

Substrate [g l−1]

Biomass [g l−1]

2,3-Butanediol [g l−1]

Acetoin [g l−1]

Ydiol/S [g g−1]

YX/S [g g−1]

Glucose

50.09 ± 0.01

4.29 ± 0.16

3.44 ± 0.10

16.78 ± 0.24

0.40 ± 0.01

0.09 ± 0.01

Glucose + fructose

51.90 ± 0.08

5.56 ± 0.33

9.41 ± 1.76

10.92 ± 1.92

0.39 ± 0.01

0.11 ± 0.01

Fructose

51.30 ± 0.28

6.98 ± 0.50

4.57 ± 0.61

14.03 ± 0.70

0.36 ± 0.04

0.14 ± 0.01

Xylose

23.24 ± 2.00

3.01 ± 0.10

5.17 ± 0.87

1.11 ± 0.09

0.27 ± 0.02

0.06 ± 0.01

Arabinose

49.24 ± 0.28

5.04 ± 0.05

12.92 ± 0.02

5.65 ± 0.08

0.38 ± 0.01

0.10 ± 0.01

Mannose

46.25 ± 1.32

5.82 ± 0.02

2.56 ± 0.26

14.74 ± 0.29

0.37 ± 0.01

0.13 ± 0.01

Sucrose

38.23 ± 0.72

7.25 ± 0.16

4.51 ± 1.12

5.45 ± 1.55

0.26 ± 0.01

0.19 ± 0.01

Molasses

52.97 ± 0.24

10.89 ± 0.14

3.04 ± 0.94

15.31 ± 1.07

0.35 ± 0.01

0.21 ± 0.01

  1. Consumed substrate, formed biomass and products as well as the diol yield (Ydiol/S) and biomass yield (YX/S) are shown
  2. Results are shown as the mean value of three replicates ± standard deviation