Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Table 4 Performance parameters of two-step pulsed fed-batch cultivations with E. coli W/445_Ediss (445_Ediss), E. coli W ∆ldhA ∆adhE ∆pta ∆frdA/445_Ediss (445_Ediss ∆4) and E. coli W ∆ldhA ∆adhE ∆pta ∆frdA ∆pykA/445_Ediss (445_Ediss ∆5) under high oxygen (DO 0–1%) and low oxygen (constant stirrer and aeration) supply

Strain	Oxygen supply	r_GLC [g l⁻¹ h⁻¹]	q_GLC [g g⁻¹ h⁻¹]	r_diol [g l⁻¹ h⁻¹]	q_diol [g g⁻¹ h⁻¹]	Y_diol/S [g g⁻¹]	Y_by-product/S [g g⁻¹]	Biomass [g l⁻¹]	2,3-Butanediol [g l⁻¹]	Acetoin [g l⁻¹]	Diol Ratio
445_Ediss	High	16.99 ± 1.60	0.84 ± 0.07	4.53 ± 0.30	0.22 ± 0.01	0.27 ± 0.01	0.08 ± 0.02	20.14 ± 0.19	37.49 ± 0.18	14.59 ± 0.03	2.57
445_Ediss	Low	5.16 ± 0.13	0.38 ± 0.03	1.61 ± 0.04	0.12 ± 0.01	0.31 ± 0.02	0.22 ± 0.05	13.76 ± 1.28	34.32 ± 1.16	13.70 ± 1.53	2.53
445_Ediss ∆4	Low	3.51 ± 0.57	0.40 ± 0.08	1.32 ± 0.21	0.15 ± 0.03	0.38 ± 0.01	0.04 ± 0.01	8.78 ± 0.29	68.12 ± 1.08	0.13 ± 0.06	627.13
445_Ediss ∆5	Low	4.28 ± 0.27	0.25 ± 0.01	1.19 ± 0.06	0.07 ± 0.01	0.28 ± 0.01	0.04 ± 0.01	16.83 ± 0.15	48.50 ± 0.03	n.d.	n.a.

Volumetric and specific substrate uptake rates, r_GLC and q_GLC, volumetric and specific diol (2,3-butanediol + acetoin) formation rates, r_diol and q_diol, diol (2,3-butanediol + acetoin) and by-product yields, average biomass, 2,3-butanediol and acetoin concentrations and ratio 2,3-butanediol:acetoin (diol ratio) are given for the production phase of each cultivation. Values for individual pulses are given in Additional file 2: Table S2
Results are shown as mean value of two replicates ± standard deviation. n.d. not detected, n.a. not applicable

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