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Table 4 Performance parameters of two-step pulsed fed-batch cultivations with E. coli W/445_Ediss (445_Ediss), E. coli W ∆ldhA ∆adhE ∆pta ∆frdA/445_Ediss (445_Ediss ∆4) and E. coli W ∆ldhA ∆adhE ∆pta ∆frdA ∆pykA/445_Ediss (445_Ediss ∆5) under high oxygen (DO 0–1%) and low oxygen (constant stirrer and aeration) supply

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Strain Oxygen supply rGLC [g l−1 h−1] qGLC [g g−1 h−1] rdiol [g l−1 h−1] qdiol [g g−1 h−1] Ydiol/S [g g−1] Yby-product/S [g g−1] Biomass [g l−1] 2,3-Butanediol [g l−1] Acetoin [g l−1] Diol
Ratio
445_Ediss High 16.99 ± 1.60 0.84 ± 0.07 4.53 ± 0.30 0.22 ± 0.01 0.27 ± 0.01 0.08 ± 0.02 20.14 ± 0.19 37.49 ± 0.18 14.59 ± 0.03 2.57
445_Ediss Low 5.16 ± 0.13 0.38 ± 0.03 1.61 ± 0.04 0.12 ± 0.01 0.31 ± 0.02 0.22 ± 0.05 13.76 ± 1.28 34.32 ± 1.16 13.70 ± 1.53 2.53
445_Ediss ∆4 Low 3.51 ± 0.57 0.40 ± 0.08 1.32 ± 0.21 0.15 ± 0.03 0.38 ± 0.01 0.04 ± 0.01 8.78 ± 0.29 68.12 ± 1.08 0.13 ± 0.06 627.13
445_Ediss ∆5 Low 4.28 ± 0.27 0.25 ± 0.01 1.19 ± 0.06 0.07 ± 0.01 0.28 ± 0.01 0.04 ± 0.01 16.83 ± 0.15 48.50 ± 0.03 n.d. n.a.
  1. Volumetric and specific substrate uptake rates, rGLC and qGLC, volumetric and specific diol (2,3-butanediol + acetoin) formation rates, rdiol and qdiol, diol (2,3-butanediol + acetoin) and by-product yields, average biomass, 2,3-butanediol and acetoin concentrations and ratio 2,3-butanediol:acetoin (diol ratio) are given for the production phase of each cultivation. Values for individual pulses are given in Additional file 2: Table S2
  2. Results are shown as mean value of two replicates ± standard deviation. n.d. not detected, n.a. not applicable