Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Table 3 Cultivation of five constructs in E. coli W using medium supplemented with 0.5% (w/v) yeast extract after 48 h

Construct	Glucose [g l⁻¹]	Biomass [g l⁻¹]	2,3-Butanediol [g l⁻¹]	Acetoin [g l⁻¹]	Acetate [g l⁻¹]	Y_diol/S [g g⁻¹]	Y_X/S [g g⁻¹]
555_Ecloa	48.07 ± 0.09	5.04 ± 0.12	2.94 ± 0.04	17.51 ± 0.07	n.d.	0.43 ± 0.01	0.10 ± 0.01
555_Koxy	32.22 ± 4.82	3.62 ± 0.23	7.14 ± 1.53	2.42 ± 0.45	6.31 ± 0.26	0.29 ± 0.02	0.11 ± 0.01
445_alsS	47.64 ± 0.17	6.27 ± 0.25	1.94 ± 0.23	18.74 ± 0.63	n.d.	0.43 ± 0.01	0.13 ± 0.01
999_Ediss	47.52 ± 0.08	5.39 ± 0.16	4.03 ± 0.24	11.49 ± 0.43	n.d.	0.33 ± 0.01	0.11 ± 0.01
445_Ediss	47.72 ± 0.15	6.33 ± 0.07	4.27 ± 0.15	14.13 ± 0.40	n. d.	0.39 ± 0.01	0.13 ± 0.01

Consumed glucose, formed biomass and metabolites as well as the diol yield (Y_diol/S) and biomass yield (Y_X/S) are shown. Designation of constructs: see Fig. 2
Results are shown as mean value of three replicates ± standard deviation. n.d. not detected

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