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Table 3 Cultivation of five constructs in E. coli W using medium supplemented with 0.5% (w/v) yeast extract after 48 h

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Construct Glucose [g l−1] Biomass [g l−1] 2,3-Butanediol [g l−1] Acetoin [g l−1] Acetate [g l−1] Ydiol/S [g g−1] YX/S [g g−1]
555_Ecloa 48.07 ± 0.09 5.04 ± 0.12 2.94 ± 0.04 17.51 ± 0.07 n.d. 0.43 ± 0.01 0.10 ± 0.01
555_Koxy 32.22 ± 4.82 3.62 ± 0.23 7.14 ± 1.53 2.42 ± 0.45 6.31 ± 0.26 0.29 ± 0.02 0.11 ± 0.01
445_alsS 47.64 ± 0.17 6.27 ± 0.25 1.94 ± 0.23 18.74 ± 0.63 n.d. 0.43 ± 0.01 0.13 ± 0.01
999_Ediss 47.52 ± 0.08 5.39 ± 0.16 4.03 ± 0.24 11.49 ± 0.43 n.d. 0.33 ± 0.01 0.11 ± 0.01
445_Ediss 47.72 ± 0.15 6.33 ± 0.07 4.27 ± 0.15 14.13 ± 0.40 n. d. 0.39 ± 0.01 0.13 ± 0.01
  1. Consumed glucose, formed biomass and metabolites as well as the diol yield (Ydiol/S) and biomass yield (YX/S) are shown. Designation of constructs: see Fig. 2
  2. Results are shown as mean value of three replicates ± standard deviation. n.d. not detected