Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Table 2 Screening result of the construct library for production of 2,3-butanediol in E. coli W after 48 h

Construct	Glucose [g l⁻¹]	Biomass [g l⁻¹]	Acetate [g l⁻¹]	Y_diol/S [g g⁻¹]	Y_X/S [g g⁻¹]
555_Ecloa	13.21 ± 0.21	1.84 ± 0.09	5.65 ± 0.07	0.07 ± 0.02	0.14 ± 0.01
445_Ecloa	50.09 ± 0.01	4.19 ± 0.43	n.d.	0.40 ± 0.01	0.08 ± 0.01
555_Koxy	36.24 ± 1.08	2.65 ± 0.22	5.78 ± 0.23	0.31 ± 0.01	0.09 ± 0.03
445_Koxy	48.98 ± 1.93	3.22 ± 0.19	3.72 ± 0.24	0.35 ± 0.01	0.07 ± 0.01
999_Koxy	27.76 ± 1.70	2.68 ± 0.06	7.86 ± 1.20	0.26 ± 0.05	0.10 ± 0.01
994_Koxy	28.88 ± 1.35	2.78 ± 0.05	7.23 ± 0.51	0.20 ± 0.02	0.10 ± 0.01
449_Koxy	50.85 ± 0.54	4.69 ± 0.28	3.12 ± 0.11	0.33 ± 0.01	0.09 ± 0.01
445_Ediss	50.09 ± 0.01	4.29 ± 0.16	n.d.	0.40 ± 0.01	0.09 ± 0.01
999_Ediss	42.11 ± 0.91	3.99 ± 0.26	4.98 ± 0.20	0.30 ± 0.01	0.09 ± 0.01
994_Ediss	39.03 ± 0.31	3.90 ± 0.11	5.51 ± 0.39	0.30 ± 0.00	0.10 ± 0.01
449_Ediss	53.36 ± 0.16	4.57 ± 0.05	n.d.	0.41 ± 0.02	0.09 ± 0.01
449_alsS	52.82 ± 0.10	3.46 ± 0.11	0.47 ± 0.10	0.34 ± 0.01	0.07 ± 0.01
945_alsS	52.95 ± 0.19	3.16 ± 0.15	1.03 ± 0.03	0.34 ± 0.00	0.06 ± 0.01
955_alsS	24.93 ± 0.18	1.86 ± 0.03	5.15 ± 0.22	0.21 ± 0.01	0.07 ± 0.01
445_alsS	20.69 ± 0.16	1.98 ± 0.18	5.71 ± 0.38	0.15 ± 0.01	0.09 ± 0.01
499_alsS	52.68 ± 0.14	2.56 ± 0.06	1.81 ± 0.20	0.34 ± 0.01	0.05 ± 0.01

Consumed glucose, produced biomass and acetate as well as the diol yield (Y_diol/S) and biomass yield (Y_X/S) are shown. Designation of constructs: see Fig. 2
Results are shown as mean value of three replicates ± standard deviation. n.d. not detected

ISSN: 1475-2859