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Table 2 Screening result of the construct library for production of 2,3-butanediol in E. coli W after 48 h

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Construct Glucose [g l−1] Biomass [g l−1] Acetate [g l−1] Ydiol/S [g g−1] YX/S [g g−1]
555_Ecloa 13.21 ± 0.21 1.84 ± 0.09 5.65 ± 0.07 0.07 ± 0.02 0.14 ± 0.01
445_Ecloa 50.09 ± 0.01 4.19 ± 0.43 n.d. 0.40 ± 0.01 0.08 ± 0.01
555_Koxy 36.24 ± 1.08 2.65 ± 0.22 5.78 ± 0.23 0.31 ± 0.01 0.09 ± 0.03
445_Koxy 48.98 ± 1.93 3.22 ± 0.19 3.72 ± 0.24 0.35 ± 0.01 0.07 ± 0.01
999_Koxy 27.76 ± 1.70 2.68 ± 0.06 7.86 ± 1.20 0.26 ± 0.05 0.10 ± 0.01
994_Koxy 28.88 ± 1.35 2.78 ± 0.05 7.23 ± 0.51 0.20 ± 0.02 0.10 ± 0.01
449_Koxy 50.85 ± 0.54 4.69 ± 0.28 3.12 ± 0.11 0.33 ± 0.01 0.09 ± 0.01
445_Ediss 50.09 ± 0.01 4.29 ± 0.16 n.d. 0.40 ± 0.01 0.09 ± 0.01
999_Ediss 42.11 ± 0.91 3.99 ± 0.26 4.98 ± 0.20 0.30 ± 0.01 0.09 ± 0.01
994_Ediss 39.03 ± 0.31 3.90 ± 0.11 5.51 ± 0.39 0.30 ± 0.00 0.10 ± 0.01
449_Ediss 53.36 ± 0.16 4.57 ± 0.05 n.d. 0.41 ± 0.02 0.09 ± 0.01
449_alsS 52.82 ± 0.10 3.46 ± 0.11 0.47 ± 0.10 0.34 ± 0.01 0.07 ± 0.01
945_alsS 52.95 ± 0.19 3.16 ± 0.15 1.03 ± 0.03 0.34 ± 0.00 0.06 ± 0.01
955_alsS 24.93 ± 0.18 1.86 ± 0.03 5.15 ± 0.22 0.21 ± 0.01 0.07 ± 0.01
445_alsS 20.69 ± 0.16 1.98 ± 0.18 5.71 ± 0.38 0.15 ± 0.01 0.09 ± 0.01
499_alsS 52.68 ± 0.14 2.56 ± 0.06 1.81 ± 0.20 0.34 ± 0.01 0.05 ± 0.01
  1. Consumed glucose, produced biomass and acetate as well as the diol yield (Ydiol/S) and biomass yield (YX/S) are shown. Designation of constructs: see Fig. 2
  2. Results are shown as mean value of three replicates ± standard deviation. n.d. not detected