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Table 1 State-of-the-art of microbial 2,3-butanediol production

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Organism Strain Over-expression Feedstock Operation mode Titer [g l−1] Yield [g g−1] Productivity [g l−1 h−1] Reference
Enterobacter aerogenes KCTC 2190 ΔldhA ΔscrR Δcra scrAB (E. aerogenes) Sugar cane molasses Fed-batch 140 0.39 2.59 [9]
Enterobacter cloacae SDM Δbdh ΔptsG Δldh ΔfrdA bdh (B. pumilus), galP (E. cloacae SDM) Glucose + fructose + CSLP Fed-batch 152 0.49 3.45 [10]
Klebsiella oxytoca ME-UD-3 aldA::Tcr   Glucose Fed-batch 130 0.48 1.63 [6]
Klebsiella pneumonia SDM   Glucose + CSLP Fed-batch 150 0.48 4.21 [7]
Saccharomyces cerevisiae D452-2 Δpdc1 Δpdc5 alsS (B. subtilis), alsD (B. subtilis), bdh1 (S. cerevisiae) Glucose + YE + peptone Fed-batch 96 0.28 0.39 [29]
Serratia marcescens H30 swrW   Sucrose + YE Fed-batch 152 0.46 2.67 [8]
Pichia pastoris X33 alsS (B. subtilis), alsD (B. subtilis) Glucose + YE Fed-batch 75 0.30 0.81 [30]
Escherichia coli MG1655 budA (K. pneumoniae), budB (K. pneumoniae), ydjL (B. subtilis) Glucose + YE Batch 31 0.38 1.69 [31]
Escherichia coli BL21(DE3) budA, budB and budC (E. cloacae subsp. dissolvens SDM) Glucose + YE Fed-batch 74 0.41 1.19 [32]
Escherichia coli W ΔldhA ΔpflB ΔadhE ΔlpdA::K.p. lpdE354 K Δmdh ΔarcA gltAR164L ilvBN (E. coli), aldB (L. lactis subsp. lactis), bdh1 (S. cerevisiae) Glucose + YE + tryptone Fed-batch 88 0.35 1.87 [33]
Escherichia coli JM109 ΔldhA Δpta ΔadhE ΔpoxB alsS (B. subtilis, alsD (B. subtilis), budC (K. pneumoniae) Glucose + YE Shake flask 14.5 0.30 0.30 [34]
Escherichia coli W budA, budB, budC (Enterobacter cloacae subsp. dissolvens) Glucose Fed-batch
High oxygen
52.1a 0.27a 4.53a This study
Escherichia coli W ΔldhA ΔadhE Δpta ΔfrdA budA, budB, budC (Enterobacter cloacae subsp. dissolvens) Glucose Fed-batch
Low oxygen
68.1 0.38 1.32 This study
Escherichia coli W budA, budB, budC (Enterobacter cloacae subsp. dissolvens) Sugar beet molasses Fed-batch 56.2 0.44 1.31 This study
  1. Comparison of 2,3-butanediol titer, yield and productivity of natural and heterologous producers from different substrates
  2. YE yeast extract, CSLP corn steep liquor powder
  3. aCombined values for 2,3-butanediol and acetoin are given (ratio 2,3-butanediol:acetoin = 2.57)