Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Erian, Anna Maria; Gibisch, Martin; Pflügl, Stefan

doi:10.1186/s12934-018-1038-0

Table 1 State-of-the-art of microbial 2,3-butanediol production

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Organism	Strain	Over-expression	Feedstock	Operation mode	Titer [g l⁻¹]	Yield [g g⁻¹]	Productivity [g l⁻¹ h⁻¹]	Reference
Enterobacter aerogenes	KCTC 2190 ΔldhA ΔscrR Δcra	scrAB (E. aerogenes)	Sugar cane molasses	Fed-batch	140	0.39	2.59	[9]
Enterobacter cloacae	SDM Δbdh ΔptsG Δldh ΔfrdA	bdh (B. pumilus), galP (E. cloacae SDM)	Glucose + fructose + CSLP	Fed-batch	152	0.49	3.45	[10]
Klebsiella oxytoca	ME-UD-3 aldA::Tcr		Glucose	Fed-batch	130	0.48	1.63	[6]
Klebsiella pneumonia	SDM		Glucose + CSLP	Fed-batch	150	0.48	4.21	[7]
Saccharomyces cerevisiae	D452-2 Δpdc1 Δpdc5	alsS (B. subtilis), alsD (B. subtilis), bdh1 (S. cerevisiae)	Glucose + YE + peptone	Fed-batch	96	0.28	0.39	[29]
Serratia marcescens	H30 swrW⁻		Sucrose + YE	Fed-batch	152	0.46	2.67	[8]
Pichia pastoris	X33	alsS (B. subtilis), alsD (B. subtilis)	Glucose + YE	Fed-batch	75	0.30	0.81	[30]
Escherichia coli	MG1655	budA (K. pneumoniae), budB (K. pneumoniae), ydjL (B. subtilis)	Glucose + YE	Batch	31	0.38	1.69	[31]
Escherichia coli	BL21(DE3)	budA, budB and budC (E. cloacae subsp. dissolvens SDM)	Glucose + YE	Fed-batch	74	0.41	1.19	[32]
Escherichia coli	W ΔldhA ΔpflB ΔadhE ΔlpdA::K.p. lpdE354 K Δmdh ΔarcA gltAR164L	ilvBN (E. coli), aldB (L. lactis subsp. lactis), bdh1 (S. cerevisiae)	Glucose + YE + tryptone	Fed-batch	88	0.35	1.87	[33]
Escherichia coli	JM109 ΔldhA Δpta ΔadhE ΔpoxB	alsS (B. subtilis, alsD (B. subtilis), budC (K. pneumoniae)	Glucose + YE	Shake flask	14.5	0.30	0.30	[34]
Escherichia coli	W	budA, budB, budC (Enterobacter cloacae subsp. dissolvens)	Glucose	Fed-batch High oxygen	52.1^a	0.27^a	4.53^a	This study
Escherichia coli	W ΔldhA ΔadhE Δpta ΔfrdA	budA, budB, budC (Enterobacter cloacae subsp. dissolvens)	Glucose	Fed-batch Low oxygen	68.1	0.38	1.32	This study
Escherichia coli	W	budA, budB, budC (Enterobacter cloacae subsp. dissolvens)	Sugar beet molasses	Fed-batch	56.2	0.44	1.31	This study

Comparison of 2,3-butanediol titer, yield and productivity of natural and heterologous producers from different substrates
YE yeast extract, CSLP corn steep liquor powder
^aCombined values for 2,3-butanediol and acetoin are given (ratio 2,3-butanediol:acetoin = 2.57)

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