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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Fig. 2

Screening result of the construct library for production of 2,3-butanediol in E. coli W after 48 h using 5% (w/v) glucose in minimal medium as substrate. Concentrations of 2,3-butanediol and acetoin obtained for the individual constructs are given as one bar (solid area = 2,3-butanediol, dashed area = acetoin). Designation of constructs as follows: Ecloa (Enterobacter cloacae subsp. cloacae DSM 30054), Koxy (Klebsiella oxytoca DSM 4798) and Ediss (Enterobacter cloacae subsp. dissolvens DSM 16657) represent gene donors for acetolactate synthase (budB), acetolactate decarboxylase (budA) and acetoin reductase/butanediol dehydrogenase (budC). alsS indicates that acetolactate synthase of B. subtilis was combined with budA and budC from Ediss. Three-digit numbers in front of donor organisms indicate promoters used for the three genes budB/alsS-budA-budC (5 = 105p, 4 = 114p and 9 = 109p)

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Microbial Cell Factories

ISSN: 1475-2859

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