Table 1 The effect of different nitrogen sources on cell growth (DCW) and recombinant DPEase activity in shake flasks
Nitrogen source | Concentration (g/L) | DCW (g/L) | DPEase activity (U/mL) |
|---|---|---|---|
Yeast extract | 15 | 4.1 ± 0.1 | 137.6 ± 2.8 |
25 | 5.2 ± 0.2 | 162.0 ± 3.4 | |
35 | 5.3 ± 0.1 | 127.7 ± 3.2 | |
Peptone | 15 | 4.1 ± 0.1 | 164.1 ± 3.5 |
25 | 5.1 ± 0.1 | 189.9 ± 2.8 | |
35 | 5.4 ± 0.2 | 186.4 ± 2.6 | |
Yeast decoction | 15 | 3.2 ± 0.1 | 122.4 ± 3.9 |
25 | 3.4 ± 0.1 | 128.4 ± 2.9 | |
35 | 4.0 ± 0.1 | 142.6 ± 3.5 | |
Corn steep powder | 5 | 2.6 ± 0.1 | 92.4 ± 1.9 |
15 | 4.3 ± 0.1 | 167.0 ± 3.3 | |
25 | 4.4 ± 0.1 | 111.1 ± 2.1 | |
35 | 5.7 ± 0.2 | 109.0 ± 2.9 | |
Cottonseed meal | 15 | 3.2 ± 0.1 | 123.8 ± 2.7 |
25 | 4.7 ± 0.2 | 199.4 ± 3.9 | |
35 | 6.2 ± 0.2 | 213.7 ± 4.8 |