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Table 1 The effect of different nitrogen sources on cell growth (DCW) and recombinant DPEase activity in shake flasks

From: Highly efficient production of Clostridium cellulolyticum H10 d-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce d-psicose

Nitrogen source Concentration (g/L) DCW (g/L) DPEase activity (U/mL)
Yeast extract 15 4.1 ± 0.1 137.6 ± 2.8
25 5.2 ± 0.2 162.0 ± 3.4
35 5.3 ± 0.1 127.7 ± 3.2
Peptone 15 4.1 ± 0.1 164.1 ± 3.5
25 5.1 ± 0.1 189.9 ± 2.8
35 5.4 ± 0.2 186.4 ± 2.6
Yeast decoction 15 3.2 ± 0.1 122.4 ± 3.9
25 3.4 ± 0.1 128.4 ± 2.9
35 4.0 ± 0.1 142.6 ± 3.5
Corn steep powder 5 2.6 ± 0.1 92.4 ± 1.9
15 4.3 ± 0.1 167.0 ± 3.3
25 4.4 ± 0.1 111.1 ± 2.1
35 5.7 ± 0.2 109.0 ± 2.9
Cottonseed meal 15 3.2 ± 0.1 123.8 ± 2.7
25 4.7 ± 0.2 199.4 ± 3.9
35 6.2 ± 0.2 213.7 ± 4.8