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Table 3 Sorbitol is better than glucose as carbon source

From: A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation

Ketone intermediate

Alcohol yield %

PBS

PBS with glucose

PBS with sorbitol

(S)-enantiomer

Talampanel ketone intermediate (compound 1)

1.25

79

87

90

MA-20565 ketone intermediate (compound 5)

2.75

37

91

100

Dolastatin ketone intermediate (compound 6)

5

23

100

96

  1. Whole-cell E. coli expressing ZRK in sorbitol containing media gives higher alcohol yields than in glucose (average yields from three experiments reported). The substrates used are as follows, talampanel ketone intermediate: 3,4-methylenedioxyphenyl acetone, MA-20565 ketone intermediate: 3–trifluoromethyl acetophenone and dolastatin ketone intermediate: 2-Phenyl-1-thiazol-2-yl-ethanone