Table 2 Stoichiometric and kinetic parameters of S. lividans growth, the recombinant glycoprotein production (APA, also known as alanine and proline-rich secreted protein or 45/47 kDa glycoprotein, an antigen from Mycobacterium tuberculosis) and RED production in conventional normal, baffled, and coiled flasks (nominal volume of 250 mL and 50 mL filling volume, incubated at 30 °C at a shaking frequency of 150 rpm). A comparison was made with the wild-type strain (WT) (Additional file 1: Figure S1)

Biomass_max (g L⁻¹)

2.5 ± 0.3

3.2 ± 0.8

3.2 ± 0.2

Biomass_max (g L⁻¹) WT

2.8 ± 0.1

4.6 ± 0.1

4.8 ± 0.1

µ (h⁻¹)

0.055 ± 0.006

0.058 ± 0.005

0.057 ± 0.005

µ (h⁻¹) WT

0.069 ± 0.005

0.091 ± 0.004

0.096 ± 0.002

k_La (h⁻¹)

41.4 ± 5.4

129.9 ± 5.0

87.4 ± 0.6

OTR_max (mmol L⁻¹ h⁻¹)

0.66 ± 0.48

9.16 ± 0.15

9.36 ± 0.28

OTR_max (mmol L⁻¹ h⁻¹) WT

~ 1.6

~ 7.2

~ 9.7

CTR_max (mmol L⁻¹ h⁻¹)

0.77 ± 0.35

5.73 ± 0.77

6.18 ± 0.41

RQ average

1.34 ± 0.49

0.69 ± 0.07

0.75 ± 0.10

UDP (mg L⁻¹)

1.540 ± 0.021

0.120 ± 0.002

0.004 ± 0.001

UDP (mg L⁻¹) WT

0.61 ± 0.11

0.19 ± 0.05

0.15 ± 0.02

GDP-mannose (ng L⁻¹)

1.1 ± 0.1

4.0 ± 1.0

6.0 ± 1.1