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Table 2 Stoichiometric and kinetic parameters of S. lividans growth, the recombinant glycoprotein production (APA, also known as alanine and proline-rich secreted protein or 45/47 kDa glycoprotein, an antigen from Mycobacterium tuberculosis) and RED production in conventional normal, baffled, and coiled flasks (nominal volume of 250 mL and 50 mL filling volume, incubated at 30 °C at a shaking frequency of 150 rpm). A comparison was made with the wild-type strain (WT) (Additional file 1: Figure S1)

From: The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks

Parameter Shake flask
Normal Coiled Baffled
Biomassmax (g L−1) 2.5 ± 0.3 3.2 ± 0.8 3.2 ± 0.2
Biomassmax (g L−1) WT 2.8 ± 0.1 4.6 ± 0.1 4.8 ± 0.1
µ (h−1) 0.055 ± 0.006 0.058 ± 0.005 0.057 ± 0.005
µ (h−1) WT 0.069 ± 0.005 0.091 ± 0.004 0.096 ± 0.002
kLa (h−1) 41.4 ± 5.4 129.9 ± 5.0 87.4 ± 0.6
OTRmax (mmol L−1 h−1) 0.66 ± 0.48 9.16 ± 0.15 9.36 ± 0.28
OTRmax (mmol L−1 h−1) WT ~ 1.6 ~ 7.2 ~ 9.7
CTRmax (mmol L−1 h−1) 0.77 ± 0.35 5.73 ± 0.77 6.18 ± 0.41
RQ average 1.34 ± 0.49 0.69 ± 0.07 0.75 ± 0.10
UDP (mg L−1) 1.540 ± 0.021 0.120 ± 0.002 0.004 ± 0.001
UDP (mg L−1) WT 0.61 ± 0.11 0.19 ± 0.05 0.15 ± 0.02
GDP-mannose (ng L−1) 1.1 ± 0.1 4.0 ± 1.0 6.0 ± 1.1
  1. The mean and standard deviation for at least three independent experiments are presented
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Microbial Cell Factories

ISSN: 1475-2859

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