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Table 1 Previously reported stoichiometric and kinetic data of recombinant glycoprotein production in S. lividans (APA, also known as alanine and proline-rich secreted protein or 45/47 kDa glycoprotein, an antigen from Mycobacterium tuberculosis) and its O-mannosylation cultured in shake flasks (nominal volume of 250 mL and 50 mL filling volume, incubated at 30 °C at a shaking frequency of 150 rpm)

From: The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks

Parameter Shake flask References
Normal Coiled Baffled
Pellet area (mm2) 2.11 ± 1.22 0.04 ± 0.02 0.02 ± 0.01 [11]
Pellet diameter (mm) 1.57 ± 0.41 0.16 ± 0.05 0.23 ± 0.06 [11]
P/Vav (WL−1) 0.20 0.44 0.51 [15]
% APA in soluble proteina 23 ± 6 15 ± 4 16 ± 5 [11]
APA produced (mgL−1)a ~ 0.51 ~ 0.81 ~ 0.81 [11]
Ytotal protein/X, g/g 0.23 ± 0.03 0.34 ± 0.03 0.37 ± 0.04 [11]
C-terminal O-mannosylation 2 5 5 [11]
  1. aCalculated based on gel densitometry performed on the soluble protein obtained