Table 1 Previously reported stoichiometric and kinetic data of recombinant glycoprotein production in S. lividans (APA, also known as alanine and proline-rich secreted protein or 45/47 kDa glycoprotein, an antigen from Mycobacterium tuberculosis) and its O-mannosylation cultured in shake flasks (nominal volume of 250 mL and 50 mL filling volume, incubated at 30 °C at a shaking frequency of 150 rpm)
Parameter | Shake flask | References | ||
|---|---|---|---|---|
Normal | Coiled | Baffled | ||
Pellet area (mm2) | 2.11 ± 1.22 | 0.04 ± 0.02 | 0.02 ± 0.01 | [11] |
Pellet diameter (mm) | 1.57 ± 0.41 | 0.16 ± 0.05 | 0.23 ± 0.06 | [11] |
P/Vav (WL−1) | 0.20 | 0.44 | 0.51 | [15] |
% APA in soluble proteina | 23 ± 6 | 15 ± 4 | 16 ± 5 | [11] |
APA produced (mgL−1)a | ~ 0.51 | ~ 0.81 | ~ 0.81 | [11] |
Ytotal protein/X, g/g | 0.23 ± 0.03 | 0.34 ± 0.03 | 0.37 ± 0.04 | [11] |
C-terminal O-mannosylation | 2 | 5 | 5 | [11] |