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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent

Fig. 4

Effect of N/nutL antitermination on expression of A/T homopolymers potent to bypass A356 deletion mutation of the mboIIM2ΔA356 gene. a Details of sequence of the selected indel mutant genes (short names). Premature stop codons are underlined. T/A homopolymer runs are marked in red. Substituted nucleotides and the position of the A356 nucleotide are in bold. b Western blot of expressed gene mutants in DH10B strain in the absence or presence (+ N) of λ phage N/nutL antitermination co-expression (both 0.1% l-arabinose induced for 1 h at 37 °C). Top and bottom panels: Equal amounts of total protein extracts from cultures harboring appropriate gene mutants were analyzed after 12.5% SDS-PAGE and immunodetection with anti-M2.MboII antibodies. Lanes 9, molecular size markers (BioRad or Fermentas, respectively), lanes 10, M2.MboII purified protein. Bands of full-length (32 kDa) and short variant of M2.MboII (14.5 kDa) are pointed by arrows. c The relative protection level of the MboII-specific sites against R.MboII digestion in plasmids bearing the corresponding mboIIM2 indel mutants

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