Fig. 3
From: Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent
Determination of the poly(A/T) minimal length requirements for transcriptional slippage. Relative fluorescence level of the rescued hybrid GFPs from the particular indel mutant gfp genes (Additional file 1: Table S3) measured after 1 h induction with 1 mM IPTG (a) or 0.1% l-arabinose (d). In-frame gfpA40, gfpA60, gfpT40 and gfpT60 fusions were used as positive controls and were taken as 100%. Error bars represent standard deviation from at least five independent determinations. Western blotting of the total extracts corresponding to the pET-based gfp genes in − 1 frame (b) and + 1 frame (c). Western blotting of the total extracts corresponding to the pBAD-based gfp genes in − 1 frame (e) and + 1 frame (f)