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Fig. 1 | Microbial Cell Factories

Fig. 1

From: CITRIC: cold-inducible translational readthrough in the chloroplast of Chlamydomonas reinhardtii using a novel temperature-sensitive transfer RNA

Fig. 1

Designs for testing different tRNATrp-UCA variants for temperature-sensitive behaviour. a Representation of the tRNA secondary structure with four variant versions annotated. Note the UCA anticodon (for UGA recognition) at the bottom of the structure. In chloroplasts, the 3′ CCA is added post-transcriptionally. Chemical modifications are not shown. b Construct design. Only the region that integrates into the chloroplast genome is shown; flanks are for homologous recombination into the genome. Each plasmid also contains an ampicillin resistance gene for selection during cloning in E. coli

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Microbial Cell Factories

ISSN: 1475-2859

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