Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

Table 2 Strain specific parameters of SuperMan₅

	SuperMan₅
max. µ_Gly (h⁻¹)	0.28 ± 0.03
Y_X/Gly (Cmol Cmol⁻¹)	0.55 ± 0.03
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{Gly}}}}\) (Cmol Cmol⁻¹)	0.47 ± 0.02
Δtime_adapt (h)	4.85

	15 °C	20 °C	25 °C	30 °C
q_MeOH (mmol g⁻¹ h⁻¹)	0.76 ± 0.12	1.17 ± 0.05	1.41 ± 0.18	1.52 ± 0.11
Y_X/MeOH (Cmol Cmol⁻¹)	0.037 ± 0.001	0.024 ± 0.002	0.022 ± 0.006	0.033 ± 0.001
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{MeOH}}}}\) (Cmol Cmol⁻¹)	0.924 ± 0.001	0.923 ± 0.048	0.961 ± 0.046	0.972 ± 0.033
C-balance	0.961 ± 0.003	0.947 ± 0.071	0.983 ± 0.074	1.005 ± 0.048
q_p (U g⁻¹ h⁻¹)	0.62 ± 0.06	0.94 ± 0.07	1.01 ± 0.06	1.05 ± 0.05

The batch phase on glycerol and the adaptation to methanol were performed at 30 °C. Subsequent methanol pulses were added at different temperatures. Parameters are defined in the footnote
max. µ_Gly maximum specific growth rate on glycerol, q_Gly specific uptake rate of glycerol during the batch, Y_X/Gly biomass yield on glycerol, \(Y_{{CO_{2} /Gly}}\) CO₂ yield on glycerol, Δtime_adapt time from first addition of methanol to a maximum in offgas activity, q_MeOH average specific uptake rate of methanol during consecutive methanol pulses, q_P specific product formation rate, Y_X/MeOH biomass yield on methanol, \(Y_{{CO_{2} /MeOH}}\) CO₂ yield on methanol, C-balance sum of biomass and CO₂ yields

ISSN: 1475-2859