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Table 2 Strain specific parameters of SuperMan5

From: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

  SuperMan5
max. µGly (h−1) 0.28 ± 0.03
YX/Gly (Cmol Cmol−1) 0.55 ± 0.03
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{Gly}}}}\) (Cmol Cmol−1) 0.47 ± 0.02
Δtimeadapt (h) 4.85
  15 °C 20 °C 25 °C 30 °C
qMeOH (mmol g−1 h−1) 0.76 ± 0.12 1.17 ± 0.05 1.41 ± 0.18 1.52 ± 0.11
YX/MeOH (Cmol Cmol−1) 0.037 ± 0.001 0.024 ± 0.002 0.022 ± 0.006 0.033 ± 0.001
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{MeOH}}}}\) (Cmol Cmol−1) 0.924 ± 0.001 0.923 ± 0.048 0.961 ± 0.046 0.972 ± 0.033
C-balance 0.961 ± 0.003 0.947 ± 0.071 0.983 ± 0.074 1.005 ± 0.048
qp (U g−1 h−1) 0.62 ± 0.06 0.94 ± 0.07 1.01 ± 0.06 1.05 ± 0.05
  1. The batch phase on glycerol and the adaptation to methanol were performed at 30 °C. Subsequent methanol pulses were added at different temperatures. Parameters are defined in the footnote
  2. max. µGly maximum specific growth rate on glycerol, qGly specific uptake rate of glycerol during the batch, YX/Gly biomass yield on glycerol, \(Y_{{CO_{2} /Gly}}\) CO2 yield on glycerol, Δtimeadapt time from first addition of methanol to a maximum in offgas activity, qMeOH average specific uptake rate of methanol during consecutive methanol pulses, qP specific product formation rate, YX/MeOH biomass yield on methanol, \(Y_{{CO_{2} /MeOH}}\) CO2 yield on methanol, C-balance sum of biomass and CO2 yields