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Table 2 Bacterial strains and plasmids used in this study

From: Identification and application of a growth-regulated promoter for improving l-valine production in Corynebacterium glutamicum

Strains and plasmids Relevant characteristicsa Source
C. glutamicum strains
 CP l-Leucine-producing, Ile, 2-TAr, SGr, LeuHxr, NVr Lab store
 ATCC13032 Wild-type strain Lab store
 ATCC 13032(pXTuf-G) C. glutamicum ATCC13032 harbouring pXTuf-G, Cmr This study
 ATCC 13032(pXC36-G) C. glutamicum ATCC13032 harbouring pXC36-G, Cmr This study
 AN02 C. glutamicum ATCC13032, ΔCgl2610::Ptuf+ilvBNXV, ΔCgl1890::Ptuf+ilvBNXV Lab store
 AN02ΔaceE C. glutamicum AN02 ΔaceE This study
 AN02ΔgltA C. glutamicum AN02 ΔgltA This study
 GRaceE C. glutamicum AN02 ΔPaceE:: PCP_2836 This study
 GRgltA C. glutamicum AN02 ΔPgltA:: PCP_2836 This study
Plasmids
 pXMJ19 Cmr; PTac promoter [30]
 pK18mobsacB Kmr; sacB [28]
 pCmGFP Carrying the sequence of green fluorescent protein (GFP) Lab store
 pXTuf-G Cmr; pXMJ19 ΔPTac::(Ptuf+GFP) This study
 pXC36-G Cmr; pXMJ19 ΔPTac::(PCP_2836+GFP) This study
 pK18-aceE Kmr; pK18mobsacB derivative containing the upstream and downstream regions of gene aceE This study
 pK18-gltA Kmr; pK18mobsacB derivative containing the upstream and downstream regions of gene gltA This study
 pK18-GRaceE Kmr; pK18mobsacB derivative containing PCP_2836 promoter covered by upstream and downstream regions of the promoter region of aceE This study
 pK18-GRgltA Kmr; pK18mobsacB derivative containing PCP_2836 promoter covered by upstream and downstream regions of the promoter region of gltA This study
  1. aIle: l-isoleucine; LeuHx: l-leucine hydroxamate; NV: norvaline; SG: sulfaguanidine; 2-TA: 2-thiazolylalanine; Cm: chloromycetin; Km: kanamycin