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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Mouse intestinal microbiota reduction favors local intestinal immunity triggered by antigens displayed in Bacillus subtilis biofilm

Fig. 2

Reduction of intestinal microbiota elicits a local humoral immune response. a Schematic schedule for the oral application of recombinant B. subtilis spores in mice. All the animals were treated with an antibiotic (ABX) cocktail for 5 days before the first application of recombinant B. subtilis spores. Three groups of six BALB/c mice each were orally provided with (i) saline solution (placebo), (ii) spores of B. subtilis tasA/sinR and (iii) spores of B. subtilis (102-207)EgTrp. The animals were orally applied with 5 × 1010 CFU per dose on days 1, 21, 42. Feces were collected daily from days 1–6 and on days 20, 41 and 50. Blood samples were collected on days 1, 21, 42 and 50. Quantification of the daily total number of spores (CFU) in feces of mice of the indicated groups after (b) day 1 and on (c) days 20, 41 and 50 post-oral application. The data represent the mean ± SEM. Determination of the local intestinal humoral immune response, fecal sIgA (d) and serological IgA (e) using indirect ELISA coated with biofilm extract of B. subtilis (102-207)EgTrp, biofilm extract of B. subtilis tasA/sinR, recombinant H6-EgTrp or recombinant H6-mCherry. The tested animal groups are indicated. The data represent the mean ± SEM, and t-test unpaired two-tailed; * p < 0.05; ** p < 0.01

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