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Table 2 Cytoplasmic GAD activities of L. brevis strains

From: Exploring the contributions of two glutamate decarboxylase isozymes in Lactobacillus brevis to acid resistance and γ-aminobutyric acid production

 

WT (U/mg of protein)

ΔgadA (U/mg of protein)

ΔgadB (U/mg of protein)

ΔgadAΔgadB (U/mg of protein)

ΔgadC (U/mg of protein)

ΔgadB: pMG36e-gadB (U/mg of protein)

EXP.

1.31 ± 0.09

1.24 ± 0.16

0.19 ± 0.08

0.04 ± 0.02

1.35 ± 0.17

3.82 ± 0.27

  1. Cells were collected at exponential phase (EXP.; 24 h) and then lysed by passing them three times through a French pressure cell press at 20,000 p.s.i., and GAD activity in the supernatant fraction was determined. One unit (U) of GAD activity was defined as the amount of enzyme that produced 1 µmol of GABA in 1 min. Specific activity is expressed as U/mg of protein
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Microbial Cell Factories

ISSN: 1475-2859

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