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Table 1 Cell-bound GAD activity

From: Exploring the contributions of two glutamate decarboxylase isozymes in Lactobacillus brevis to acid resistance and γ-aminobutyric acid production

 

WT (U/g DCW)

ΔgadA (U/g DCW)

ΔgadB (U/g DCW)

ΔgadAB (U/g DCW)

ΔgadC (U/g DCW)

EXP.

218.13 ± 23.18

173.56 ± 8.75

13.44 ± 2.67

0.05 ± 0.02

0.75 ± 0.09

EARLY-STAT.

672.72 ± 22.04

605.47 ± 30.12

22.49 ± 7.43

0.08 ± 0.07

1.96 ± 0.14

  1. Cells were collected at the exponential phase (EXP.; 12 h) and early stationary phase (EARLY-STAT.; 30 h). One unit (U) of GAD activity was defined as the mass of cells that produced 1 µmol of GABA in 1 min. Specific activity was defined as U/g dry cell weight (DCW) of cells
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Microbial Cell Factories

ISSN: 1475-2859

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