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Table 2 List of peptides sequenced in LC–MS/MS experiments for upper and bottom band

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Start–end Sequence Modifications # Missed cleavages Upper band Bottom band
# PSMs Ion scorea # PSMs Ion scorea
37–45 VAAHITGTR   0 9 40 1 25
48–57 SNTLSSPNSK   0 4 39 1 38
65–73 KINSWESSR   1 28 64 26 64
66–73 INSWESSR   0 3 46 3 46
74–85 SGHSFLSNLHLR   0 105 69 85 64
86–94 NGELVIHEK   0 22 63 20 63
95–106 GFYYIYSQTYFR   0 16 52 15 66
95–112 FQEEIK   0 2 24 2 24
107–116 FQEEIKENTK   1 47 59 45 57
113–127 NDKQmVQYIYK M5 (oxidation) 1 31 63 5 65
117–127 NDKQMVQYIYK   1 1 49 25 63
120–127 QmVQYIYK M2 (oxidation) 0 22 47 21 48
120–127 QMVQYIYK   0 5 44 5 44
120–139 QmVQYIYKYTSYPDPILLmK M2 (oxidation); M19 (oxidation) 1 1 40 0 0
128–139 YTSYPDPILLmK M11 (oxidation) 0 41 53 43 54
128–139 YTSYPDPILLMK   0 5 44 12 45
128–142 YTSYPDPILLmKSAR M11 (oxidation) 1 1 38 3 33
143–166 DAEYGLYSIYQGGIFELK   0 20 78 20 84
143–170 DAEYGLYSIYQGGIFELKENDR   1 7 92 9 105
171–196 IFVSVTNEHLIDmDHEASFFGAFLVG M13 (oxidation) 0 2 30 2 46
  1. aIons score is − 10*Log(P) where p is the probability that the observed peptide match is a random event
  2. Peptides identified in bottom band based on a single peptide-spectrum match (PMS) are marked in italics