Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Table 2 List of peptides sequenced in LC–MS/MS experiments for upper and bottom band

Start–end	Sequence	Modifications	# Missed cleavages	Upper band		Bottom band
Start–end	Sequence	Modifications	# Missed cleavages	# PSMs	Ion score^a	# PSMs	Ion score^a
37–45	VAAHITGTR		0	9	40	1	25
48–57	SNTLSSPNSK		0	4	39	1	38
65–73	KINSWESSR		1	28	64	26	64
66–73	INSWESSR		0	3	46	3	46
74–85	SGHSFLSNLHLR		0	105	69	85	64
86–94	NGELVIHEK		0	22	63	20	63
95–106	GFYYIYSQTYFR		0	16	52	15	66
95–112	FQEEIK		0	2	24	2	24
107–116	FQEEIKENTK		1	47	59	45	57
113–127	NDKQmVQYIYK	M5 (oxidation)	1	31	63	5	65
117–127	NDKQMVQYIYK		1	1	49	25	63
120–127	QmVQYIYK	M2 (oxidation)	0	22	47	21	48
120–127	QMVQYIYK		0	5	44	5	44
120–139	QmVQYIYKYTSYPDPILLmK	M2 (oxidation); M19 (oxidation)	1	1	40	0	0
128–139	YTSYPDPILLmK	M11 (oxidation)	0	41	53	43	54
128–139	YTSYPDPILLMK		0	5	44	12	45
128–142	YTSYPDPILLmKSAR	M11 (oxidation)	1	1	38	3	33
143–166	DAEYGLYSIYQGGIFELK		0	20	78	20	84
143–170	DAEYGLYSIYQGGIFELKENDR		1	7	92	9	105
171–196	IFVSVTNEHLIDmDHEASFFGAFLVG	M13 (oxidation)	0	2	30	2	46

^aIons score is − 10*Log(P) where p is the probability that the observed peptide match is a random event
Peptides identified in bottom band based on a single peptide-spectrum match (PMS) are marked in italics

ISSN: 1475-2859