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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 7

Effect of aprotynin on hsTRAIL secretion by L. lactis (hsTRAIL+). The presence of aprotynin during induction of hsTRAIL expression and secretion did not affect proteolysis of secreted hsTRAIL as detected by Western blot. M—molecular weight marker; rhTRAIL (PeproTech)—positive control; NC—protein sample precipitated from supernatant of L. lactis (hsTRAIL−) culture as the negative control; 0; 2 and 5 (µg/ml)—concentrations of aprotynin used during induction of protein expression with nisin (25 ng/ml). Data from one representative experiment out of five performed are shown

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Microbial Cell Factories

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