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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 3

Optimization of growth conditions for L. lactis (hsTRAIL+) bacteria. L. lactis (hsTRAIL+) bacteria were cultured in M17 broth medium supplemented with chloramphenicol (10 µg/ml) and different concentration of glucose or/and l-arginine, as the source of energy. After 4 and 24 h of culture, pH (y-axis) and optical density at 600 nm (OD600; indicated by the numbers) were measured. For further experiments, the combination of glucose and l-arginine at 0.3% both, was selected. Experiment was performed three times, each in triplicates and mean values ± SD are presented

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