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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 2

Selection of L. lactis (hsTRAIL+) clones. Left—results of DNA electrophoretic separation of PCR reaction products. As templates for PCR reaction, plasmids isolated from L. lactis recombinant clones (∆1–∆6), were used. White arrow- sequence for hsTRAIL (438 bp) presents in selected L. lactis clone ∆3. Right—results from DNA electrophoresis for hsTRAIL-insert after digestion of isolated plasmids with EcoRV and XbaI (on the left) and after PCR reaction with digested inserts used as the templates (on the right). White arrow—sequence for hsTRAIL (438 bp) presents in selected L. lactis clone ∆3. M-DNA molecular weight marker; V124—plasmid vector pNZ8124 without hsTRAIL-insert (negative control)

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