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Fig. 11 | Microbial Cell Factories

Fig. 11

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 11

Induction of apoptosis of colon cancer HCT116 cells by hsTRAIL from supernatant of L. lactis (hsTRAIL+). HCT116 cells were treated with supernatants from broth cultures of L. lactis (hsTRAIL±) bacteria and rhTRAIL (positive control) in a corresponding dose of 100 ng/ml for 48 h and then stained with FITC-Annexin V and PI to determine cancer cells apoptosis. HCT116 turned apoptotic after treatment with supernatant of L. lactis (hsTRAIL+) bacteria (a, d) in contrast to supernatant of L. lactis (hsTRAIL−) (negative control; c) or medium (b). As positive control, standard TRAIL formulation (rhTRAIL, Peprotech) in corresponding concentration was used (e). Dot-plots. FL1-H—FITC; FL2-H—PI. The bars (a) indicate the mean value ± SD of three independent experiments, each performed in triplicates. Differences between the groups were calculated using two-way ANOVA with Tukey’s multiple comparisons post-test. *p < 0.05

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