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Fig. 10 | Microbial Cell Factories

Fig. 10

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 10

Lactococcus lactis-derived hsTRAIL-induced death of human colon cancer HCT116 cells in vitro. HCT116 cells were incubated for 48 h with increasing concentrations of hsTRAIL in supernatants from broth culture of L. lactis (hsTRAIL+). The following controls in experimental setup were used: corresponding volumes of supernatants of L. lactis (hsTRAIL−)—negative control; corresponding concentrations of recombinant human TRAIL (Peprotech)—positive control. Viability of cancer cells was assessed by MTS assay. Results are presented as % of viability of cells incubated in standard culture medium only. hsTRAIL present in supernatant from L. lactis (hsTRAIL+) broth culture decreased viability of cancer cells in a dose-dependent manner, comparable to corresponding concentrations of positive control. Table below—concentration of TRAIL [ng/ml] in specified volume of supernatant or concentration of standard TRAIL formulation (rhTRAIL, Peprotech). The bars indicate the mean value ± SD of three independent experiments, each performed in triplicates. Statistical significance was calculated using two-way ANOVA with Tukey’s multiple comparison post-test. *p < 0.05, **p < 0.01, ***p < 0.001

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