Fig. 4From: Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeastEffect of CYP76AH15 mutants on the synthesis of ferruginol in miltiradiene producing yeast strains. a GC–MS chromatograms of control MILT(−) strains expressing CfTPS1, CfTPS3 and CfPOR able to produce miltiradiene (4) and abietatriene (5). Expression of native CYP76AH15 lead to production of 6 which corresponds to ferruginol according to its comparison with an authentic standard (STD), retention time and fragmentation pattern. b Enhanced production of 6 from CYP76AH15 mutants, altered in the SRS6 region. c Chemical structures of the identified products produced by the yeast strains mentioned in aBack to article page