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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast

Fig. 4

Effect of CYP76AH15 mutants on the synthesis of ferruginol in miltiradiene producing yeast strains. a GC–MS chromatograms of control MILT(−) strains expressing CfTPS1, CfTPS3 and CfPOR able to produce miltiradiene (4) and abietatriene (5). Expression of native CYP76AH15 lead to production of 6 which corresponds to ferruginol according to its comparison with an authentic standard (STD), retention time and fragmentation pattern. b Enhanced production of 6 from CYP76AH15 mutants, altered in the SRS6 region. c Chemical structures of the identified products produced by the yeast strains mentioned in a

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