Fig. 3From: Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeastDiterpenoid product profiles from the CYP76AH15 mutant variants, in comparison to the native enzyme when expressed in yeast cells producing 13R-manoyl oxide (MO). a GC–MS chromatograms of the control strain MO (−) with CfPOR only, and strains from CYP76AH15 mutant-variants at the SRS1, SRS3 and SRS5 regions showing enhanced production of 11-oxo-13R-manoyl oxide (2). b Relative yields of the diterpenes 1 (red bars), 2 (green bars), 3 (light purple bars) and unknown 7 (light pink bars). The yeast strains expressing the SRS1, SRS3 and SRS5 variants of CYP76AH15 produced significantly higher levels of 2 and 3 and lower levels of 7, in comparison to the native enzyme. c Chemical structures of the identified products formed by yeast. d GC–MS chromatograms of the control strain MO (−) with CfPOR only, native CYP76AH15, SRS5 and SRS6 mutated variants, and 11β-hydroxy-MO as internal standard (STD). e MS spectrum of (7) with parent ion m/z 320. IS internal standard (1 mg/L 1-eicosene), MO 13R-manoyl oxide, TIC total ion chromatogram. Error bars indicate standard deviation from three biological replicatesBack to article page