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Table 1 Strains and plasmids used in this study

From: Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe

Strain Genotype Reference/source
12804 h90 ura4-D18 ade6-M210 leu1-32 YGRC/NBRP
12804 ku70Δ h90 ura4-D18 ade6-M210 leu1-32 ku70Δ Ozaki et al. [17]
SPO-01 12804 ku70Δ, isp6Δ::Pcam1-acc, integration of mcr gene at leu This study
SPO-02 12804 ku70Δ, isp6Δ::Pcam1-acc, ppp16Δ::Pef1a-c-mcr-C*, adh8Δ::Pef1-a-c-mcr-C*-Phsp-mcr-N This study
SPO-03 SPO-02, atg4Δ::Pcam1-acsSE*, adh4Δ::Pcam1-atd1 This study
SPO-04 SPO-03, integration of Pcam1-ptk1 at leu This study
SPO-05 SPO-04, gut2Δ::Phsp-SPBC359.04c-BGL This study
SPO-06 SPO-05, aap1Δ::Pcam1-atd1, fma2Δ::Pcam1-acsSE* This study
SPO-07 SPO-06, sxa2Δ::Pef1a-c-mcrC* This study
Plasmid Description Reference/source
pDUAL-FFH31 Vector under cam1 promoter control RIKEN BRC [37]
pDUAL-FFH61 Vector under ef1-a-c promoter control RIKEN BRC [37]
pDUAL-FFH1 Vector under nmt1 promoter control RIKEN BRC [37]
pDUAL-hsp-SPBC359.04c-BGL Vector for cell surface-display expression of protein fusion of BGL from Aspergillus aculeatus with SPBC359.04c anchor protein Ozaki et al. [17]
pDUAL-FFH1-MCR Vector for expression of mcr from Chloroflexus aurantiacus This study
pDUAL-FFH61-MCR-C Vector for expression of mcr-C* from Chloroflexus aurantiacus This study
pDUAL-hsp-MCR-N Vector for expression of mcr-N from Chloroflexus aurantiacus This study
pDUAL-FFH31-ACC Vector for expression of cut6 from Schizosaccharomyces pombe This study
pDUAL-FFH31-ACS Vector for expression of acsSE* from Salmonella enterica This study
pDUAL-FFH31-ACS1 Vector for expression of acs1 from Schizosaccharomyces pombe This study
pDUAL-FFH31-ATD1 Vector for expression of atd1 from Schizosaccharomyces pombe This study
pDUAL-FFH31-PTK1 Vector for expression of ptk1 from Schizosaccharomyces pombe This study
pMZ374 Vector for expressing adh1:cas9/rrk1:sgRNA in fission yeast: empty sgRNA target Addgene (#59896)
pMZ374-aap1-1266 pMZ374 derivative, aap1(1266) sgRNA target This study
pMZ374-atg4-46 pMZ374 derivative, atg4(46) sgRNA target This study
pMZ374-fma2-950 pMZ374 derivative, fma2(950) sgRNA target This study
pMZ374-isp6-1069 pMZ374 derivative, isp6(1069) sgRNA target This study
pMZ374-ppp16-83 pMZ374 derivative, ppp16(83) sgRNA target This study
pMZ374-sxa2-1436 pMZ374 derivative, sxa2(1436) sgRNA target This study
pMZ374-adh4-100 pMZ374 derivative, adh4(100) sgRNA target This study
pMZ374-adh8-434 pMZ374 derivative, adh8(434) sgRNA target Ozaki et al. [17]
pMZ374-gut2-1749 pMZ374 derivative, gut2(1749) sgRNA target Ozaki et al. [17]
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Microbial Cell Factories

ISSN: 1475-2859

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