Table 1 Strains and plasmids used in this study
Strain | Genotype | Reference/source |
|---|---|---|
12804 | h90 ura4-D18 ade6-M210 leu1-32 | YGRC/NBRP |
12804 ku70Δ | h90 ura4-D18 ade6-M210 leu1-32 ku70Δ | Ozaki et al. [17] |
SPO-01 | 12804 ku70Δ, isp6Δ::Pcam1-acc, integration of mcr gene at leu | This study |
SPO-02 | 12804 ku70Δ, isp6Δ::Pcam1-acc, ppp16Δ::Pef1a-c-mcr-C*, adh8Δ::Pef1-a-c-mcr-C*-Phsp-mcr-N | This study |
SPO-03 | SPO-02, atg4Δ::Pcam1-acsSE*, adh4Δ::Pcam1-atd1 | This study |
SPO-04 | SPO-03, integration of Pcam1-ptk1 at leu | This study |
SPO-05 | SPO-04, gut2Δ::Phsp-SPBC359.04c-BGL | This study |
SPO-06 | SPO-05, aap1Δ::Pcam1-atd1, fma2Δ::Pcam1-acsSE* | This study |
SPO-07 | SPO-06, sxa2Δ::Pef1a-c-mcrC* | This study |
Plasmid | Description | Reference/source |
|---|---|---|
pDUAL-FFH31 | Vector under cam1 promoter control | RIKEN BRC [37] |
pDUAL-FFH61 | Vector under ef1-a-c promoter control | RIKEN BRC [37] |
pDUAL-FFH1 | Vector under nmt1 promoter control | RIKEN BRC [37] |
pDUAL-hsp-SPBC359.04c-BGL | Vector for cell surface-display expression of protein fusion of BGL from Aspergillus aculeatus with SPBC359.04c anchor protein | Ozaki et al. [17] |
pDUAL-FFH1-MCR | Vector for expression of mcr from Chloroflexus aurantiacus | This study |
pDUAL-FFH61-MCR-C | Vector for expression of mcr-C* from Chloroflexus aurantiacus | This study |
pDUAL-hsp-MCR-N | Vector for expression of mcr-N from Chloroflexus aurantiacus | This study |
pDUAL-FFH31-ACC | Vector for expression of cut6 from Schizosaccharomyces pombe | This study |
pDUAL-FFH31-ACS | Vector for expression of acsSE* from Salmonella enterica | This study |
pDUAL-FFH31-ACS1 | Vector for expression of acs1 from Schizosaccharomyces pombe | This study |
pDUAL-FFH31-ATD1 | Vector for expression of atd1 from Schizosaccharomyces pombe | This study |
pDUAL-FFH31-PTK1 | Vector for expression of ptk1 from Schizosaccharomyces pombe | This study |
pMZ374 | Vector for expressing adh1:cas9/rrk1:sgRNA in fission yeast: empty sgRNA target | Addgene (#59896) |
pMZ374-aap1-1266 | pMZ374 derivative, aap1(1266) sgRNA target | This study |
pMZ374-atg4-46 | pMZ374 derivative, atg4(46) sgRNA target | This study |
pMZ374-fma2-950 | pMZ374 derivative, fma2(950) sgRNA target | This study |
pMZ374-isp6-1069 | pMZ374 derivative, isp6(1069) sgRNA target | This study |
pMZ374-ppp16-83 | pMZ374 derivative, ppp16(83) sgRNA target | This study |
pMZ374-sxa2-1436 | pMZ374 derivative, sxa2(1436) sgRNA target | This study |
pMZ374-adh4-100 | pMZ374 derivative, adh4(100) sgRNA target | This study |
pMZ374-adh8-434 | pMZ374 derivative, adh8(434) sgRNA target | Ozaki et al. [17] |
pMZ374-gut2-1749 | pMZ374 derivative, gut2(1749) sgRNA target | Ozaki et al. [17] |