Fig. 3
From: Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3
A summary of the steps in the genetic and metabolic engineering of P. chlororaphis for arbutin production. a The construction step for enhanced arbutin production; b growth profile of the arbutin-producing transformants harboring plasmid; c the amount of arbutin produced by transformants harboring plasmid checked at 48 h. B, P3-Ar3-pBBR-aroB; C, P3-Ar3-pBBR-phzC; D, P3-Ar3-pBBR-aroD; E, P3-Ar3-pBBR-aroE; P, P3-Ar3-pBBR-ppsA; T, P3-Ar3-pBBR-tktA; X, P3-Ar3-pBBR-XanB2; XC, P3-Ar3-pBBR-XanB2–phzC; MA, P3-Ar3-pBBR-MNX1–AS; CK, P3-Ar3.; d the yield of arbutin produced by engineered P. chlororaphis at 48 h. Data are presented as the mean ± standard deviation of three independent experiments (n = 3)