Fig. 7

Cephalosporin C production of WT, ∆Acatg8 and ∆Acatg8/xyl^P-GFP-Atg8 in the modified MDFA medium supplemented with 1% xylose. a Growth of WT, ∆Acatg8 and ∆Acatg8/pAg::xyl^P-GFP-Acatg8-T during fermentation. The fungal mycelium dry weight was determined after drying at 42 °C in a hot air oven until a constant weight. ∆Acatg8/pAg::xyl^P-GFP-Acatg8-T, ∆Acatg8 was complemented with Acatg8 under the xylose inducible promoter xyl^P. b Cephalosporin C production of WT, ∆Acatg8 and ∆Acatg8/pAg::xyl^P-GFP-Acatg8-T. Cephalosporin C production was determined by bioassays against B. subtilis CGMCC 1.1630. 40 μl of culture filtrates of fermentation was used to detect the cephalosporin C production. The plate was added 50,000 units of penicillinase to exclude penicillin in culture filtrates. Error bars show standard deviations of three independent experiments