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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhancing the production of cephalosporin C through modulating the autophagic process of Acremonium chrysogenum

Fig. 1

Identification of an ATG8 gene homologue from A. chrysogenum. a Acatg8 with 2 introns. b Sequence alignment of AcAtg8 with its homologs. AcAtg8 shows 96% identity to Atg8 from A. oryzae, 83% identity to Atg8 from U. maydis, 69% identity to Atg8 from D. discoideum, 91% identity to Atg8 from M. oryzae, 78% identity to Atg8 from S. cerevisiae. The asterix indicates the glycine cutting site that is conserved at the C terminal. c Viability of ∆atg8 and its complemented strains. The viability of ∆atg8 and its complemented strains was detected after 18 days of incubation on the nitrogen-starved medium (SG-N). d Distribution of AcAtg8 in A. chrysogenum. Fluorescence observation demonstrated that AcAtg8 was widely distributed throughout the hyphae when the fungal strains grew under nutrient-rich conditions (Nonstarvation), while AcAtg8 was transferred into vacuoles under starvation condition (starvation). WT: the S. cerevisiae wild-type strain; ∆atg8: the S. cerevisiae ATG8 mutant; YC1-3: the complemented strains of ∆atg8; ∆atg8/pYES2: ∆atg8 carrying the plasmid pYES2 as the control; DIC; differential interference contrast; GFP: green fluorescent protein

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