Fig. 6

Overexpression of Kex2 increased cleavage efficiency of furin cleavage sites in Pichia pastoris. a Genomic PCR for analyzing integration of Kex2 gene into GS115 genome using 5′ GAP general upstream and Kex2 specific downstream primers. b Kex2 overexpression analysis in GS115-Kex2 strains by RT-PCR. Expression of β-actin was used as control. c Western blot results of GS115 strains. The western blot samples were treated with Endo Hf. d Western blot results of GS115-Kex2 strains. The western blot samples were treated with Endo Hf. e Quantitative western blot analysis of cleavage efficiency of three furin cleavage sites in GS115 and GS115-Kex2 strains. *p < 0.05, ***p < 0.001