Fig. 5

Heterologous expression of furin increased cleavage efficiency of furin cleavage sites in Pichia pastoris. a Genomic PCR for analyzing integration of furin gene into GS115 genome using AOX1 general primers. b Expression analysis of integrated furin gene in Pichia pastoris GS115 by RT-PCR with β-actin and furin specific primers. c Western blot results of GS115 strains. The western blot samples were treated with Endo Hf. d Western blot results of GS115-furin strains. The western blot samples were treated with Endo Hf. e Quantitative western blot analysis of cleavage efficiency of three furin cleavage sites. *p < 0.05, ***p < 0.001