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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris

Fig. 1

Cleavage of furin cleavage site in Pichia pastoris. a Schematic diagram of expression vectors using in this study. b Western blot of expressed products using anti-GFP antibody, the culture supernatants didn’t be treated with Endo Hf endoglycosidase which cleaves the chitobiose core of high mannose from N-linked glycoprotein. c Western blot of expressed products using anti-GFP antibody, the culture supernatants were treated with Endo Hf to show α-factor-GFP fusion protein after high mannose chains were removed from α-factor peptide. d Quantitative analysis of the western blot results, compared GFP with GFP and α-factor-GFP

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