Table 2 Transformation of C. reinhardtii
From: Biomass from microalgae: the potential of domestication towards sustainable biofactories
Organelle | DNA-delivery method | Genetic mechanism | Advantages | Disadvantages | Selection marker |
|---|---|---|---|---|---|
Nucleus | Electroporation gene-gun bombarment A. tumefaciens-mediated Glass beads Silicon carbide whiskers and aminoclay nanoparticles | Ectopic recombination (random integration) | Protein can be expressed as secreted protein Post-translational modifications | Identification of high-expressing Transformants gene silencing | Resistance to Paromycin, Zeocin, Hygromycin, Chloramphenicol Auxotrophic complementation (ARG7, NIT1, oee1) |
Chloroplast | Gene-gun bombarment Glass beads | Homologous recombination (site-specific integration) | Compartmentalization Lacks gene silencing High expression level | Need to identify homoplasmic transformant Lacks post-translational modifications | resistance to Spectinomycin Auxotrophic complementation (atpB, psbH) |