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Table 3 FHT production in dependence on inducer (IPTG) concentration and growth rate

From: E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)

µ (h−1)

qs,glu (g/g/h)

IPTG—conc. (mM)

Specific product titer (mgP/gX)

0.08 ± 0.014

0.29 ± 0.030

0.05

41 ± 1.3

0.07 ± 0.015

0.28 ± 0.046

0.5

38 ± 0.9

0.05 ± 0.004

0.16 ± 0.0013

0.5

35 ± 1.1

0.02 ± 0.006

0.066 ± 0.0009

0.5

27 ± 0.7

  1. Analysis of productivity for FHT (expressed as soluble protein) in dependence on inducer (IPTG) concentration and growth rate (specific glucose uptake rate respectively) in HMS174(DE3) cultivations. FHT production does not decrease with less inducer but is lower with less glucose supplied. Standard deviations were evaluated from triple measurements and calculated by error propagation