E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)

Table 2 FHT and CH3H production in dependence on specific lactose uptake rates

FHT—expressed as soluble protein			CH3H—expressed as inclusion body
µ (h⁻¹)	q_s,lac (g/g/h)	Specific product titer (mg_P/g_X)	µ (h⁻¹)	q_s,lac (g/g/h)	Specific product titer (mg_P/g_X)
0.07 ± 0.015	0.23 ± 0.034	27 ± 0.9	0.08 ± 0.021	0.23 ± 0.038	390 ± 31
0.04 ± 0.014	0.12 ± 0.004	17 ± 1.2	0.04 ± 0.012	0.13 ± 0.005	240 ± 16
0.02 ± 0.001	0.055 ± 0.004	11 ± 0.3	0.02 ± 0.009	0.074 ± 0.012	15 ± 2
			0.01 ± 0.004	0.029 ± 0.006	nd

Analysis of productivity for FHT (expressed as soluble protein) and CH3H (expressed as inclusion body) in dependence on the specific lactose uptake rate in HMS174(DE3) cultivations. Standard deviations were evaluated from triple measurements and calculated by error propagation

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