Fig. 2

Initial cultivation of HMS174(DE3) on lactose. During the first 4 h (dark grey area) the glucose feed was still running [specific glucose uptake rate = 0.27 g/g/h (white squares)] and lactose was pulsed to 10 g/L (grey diamonds) allowing the cells to adapt to lactose. After 4 h, the glucose feed was switched off, however lactose was still present in excess (light grey area), showing a maximum specific lactose uptake rate (black circles) of 0.23 g/g/h in the absence of glucose. After 8 h of induction, lactose was no longer present in excess