Fig. 1From: E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)Sugar concentrations in shake-flasks cultivations of a BL21(DE3), b JM109(DE3) and c HMS174(DE3). Sugar-concentrations over time in the supernatant of shake-flask cultivations on DeLisa minimal media with 9 g/L glucose (white crosses) and 9 g/L lactose (grey diamonds). Three cultivations of a BL21(DE3), b JM109(DE3) and c HMS174(DE3) are compared. As BL21(DE3) was the only Gal− strain, accumulation of galactose (dark grey triangles) was observed when lactose was being consumed. The error on sugar measurements is on average 5%Back to article page