Fig. 7
From: High level in vivo mucin-type glycosylation in Escherichia coli
UPLC-ESI-QTOF-MS analysis of intact unglycosylated and glycosylated T7Muc10. Purified protein samples were dialysed, filtered, and analysed by UPLC-ESI-QTOF mass spectrometry. Charge deconvolution of MS spectra was realized using the MaxEnt 1 algorithm. Charge-deconvoluted MS spectra of intact N-terminally truncated T7Muc10 (AS 21–159) (a) and glycosylated T7Muc10 (b) are shown. The average molecular mass (Da) of respective proteoforms is given. Each of the up to 24 O-linked β-N-acetylgalactosamines results in a mass increment of 203.2 Da