Fig. 4
From: High level in vivo mucin-type glycosylation in Escherichia coli
Primary structure of T7Muc10. In silico proteolytic (Asp-N) digestion of truncated T7Muc10 results in nine peptides (1–9) with four different sequences. The repetitive amino acid sequences EA2 and EAN are highlighted. The N-terminal peptide sequence comprising amino acids 1 to 20 could not be covered by HPLC-ESI-LTQ-OT-analysis (shown in bold and grey). Potential glycosylation sites (Ser/Thr) are highlighted in red. Predicted trypsin cleavage sites are indicated with an asterisk